Start from a clear question
Start from a clear question
Reference samples should be regularly injected along the sequence.
They measure analytical stability because the sample itself is the same.
QC samples should be representative of the chamical complexity of the samples
Pooled samples are often the preferred choice
Experiment combining full scan and fragmentation experiment are quite popular, because fragmentation patterns are needed for metabolite annotation and for setting-up quantitative methods
The more experiments I do, the worst is my estimate of the peak area …